Search results for "Gordonia Bacterium"

showing 2 items of 2 documents

Degradation of long-chain n-alkanes in soil microcosms by two actinobacteria

2012

The ability of two recently isolated actinobacteria, that degrade medium and long chain n-alkanes in laboratory water medium, was investigated in soil microcosms using different standard soils that were artificially contaminated with n-alkanes of different length (C(12)- C(20)- C(24)- C(30)). The two strains, identified as Nocardia sp. SoB and Gordonia sp. SoCp, revealed a similar high HC degradation efficiency with an average of 75% alkane degraded after 28 days incubation. A selectivity of bacteria towards n-alkanes of different length was detected as well as a consistent effect of soil texture and other soil physical chemical characteristics on degradation. It was demonstrated the specif…

Environmental Engineeringfood.ingredientSoil textureSettore AGR/13 - Chimica AgrariaGeneral MedicineGordoniaBiologybiology.organism_classificationSettore BIO/19 - Microbiologia GeneraleNocardiaActinobacteriaMicrobiologyfoodBioremediationBiodegradation EnvironmentalEnvironmental chemistrySoil waterAlkanesDegradation (geology)Soil PollutantsMicrocosmGordonia BacteriumBacteriaSoil MicrobiologyBioremediation soil microcosms GC-MS n-alkanes Nocardia Gordonia.
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Involvement of an Alkane Hydroxylase System of Gordonia sp. Strain SoCg in Degradation of Solid n-Alkanes▿

2010

ABSTRACT Enzymes involved in oxidation of long-chain n -alkanes are still not well known, especially those in Gram-positive bacteria. This work describes the alkane degradation system of the n -alkane degrader actinobacterium Gordonia sp. strain SoCg, which is able to grow on n -alkanes from dodecane (C 12 ) to hexatriacontane (C 36 ) as the sole C source. SoCg harbors in its chromosome a single alk locus carrying six open reading frames (ORFs), which shows 78 to 79% identity with the alkane hydroxylase (AH)-encoding systems of other alkane-degrading actinobacteria. Quantitative reverse transcription-PCR showed that the genes encoding AlkB (alkane 1-monooxygenase), RubA3 (rubredoxin), RubA4…

food.ingredientMutantMolecular Sequence DataAlkBGene ExpressionStreptomyces coelicolorGordoniaLong-chain n-alkaneGordoniaSettore BIO/19 - Microbiologia Generalemedicine.disease_causeApplied Microbiology and BiotechnologyPolymerase Chain ReactionGas Chromatography-Mass SpectrometryfoodRubredoxinAlkanesSPME/GC-MSmedicineEscherichia coliNADH NADPH OxidoreductasesGordonia BacteriumEscherichia coliBiotransformationSequence DeletionEcologybiologyReverse Transcriptase Polymerase Chain ReactionRubredoxinsStreptomyces coelicolorGordonia BacteriumSequence Analysis DNAbiology.organism_classificationCarbonalkane hydroxylase AlkBBiochemistrybiology.proteinBiodegradationCytochrome P-450 CYP4AFatty AlcoholsBacteriaFood ScienceBiotechnology
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